规格
|
Concentration:
|
200 U⁄µl |
Format:
|
Tube(s) |
No. of Reactions:
|
200 reactions, 200 Reactions |
Quantity:
|
200 reactions |
Sensitivity:
|
Medium |
Enzyme Function:
|
RNA dependent DNA Polymerase |
Size (Final Product):
|
12.3 kb or less |
Form:
|
Frozen |
Optimal Reaction Temperature:
|
50° C |
Reverse Transcriptase:
|
SuperScript™ III |
Ribonuclease H Activity:
|
Reduced |
Template:
|
ssRNA |
Product Line:
|
SuperScript™ |
储存
|
SuperScript® III Reverse Transcriptase (4 × 10,000 units total, at 200U/µL) is supplied with a vial (4 × 1 mL) of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial (4 × 500 µL) of 100 mM DTT. Store at -20°C. |
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) that was created by introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich target RNAs, and overall better performance than wild-type MMLV and MMLV RNase H-minus enzymes.
SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.
Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.