- 商品介绍
- 规格参数
- 包装参数
规格 |
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Final Product Type: | cDNA (First-Strand) |
For Use With (Application): | Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR) |
Includes: | 10X RT Buffer (1mL); 10X RT Random Primers (1mL); 25X dNTP Mix (100mM, 0.2mL); MultiScribe Reverse Transcriptase (0.2mL at 50U/μL); RNase Inhibitor (2 × 0.1mL at 20U/μL) |
Quantity: | 200 reactions |
Sample Type: | RNA |
Size (Final Product): | 7 kb or less |
Optimal Reaction Temperature: | 37° C |
Reaction Format: | Separate Components |
Reverse Transcriptase: | MultiScribe™ |
储存 |
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Contents: • 10X RT Buffer (1 mL) • 10X RT Random Primers (1 mL) • 25X dNTP Mix (100 mM, 0.2 mL) • MultiScribe® Reverse Transcriptase (0.2 mL at 50 U/µL) • RNase Inhibitor (1 × 0.2 mL at 20 U/µL) Store between -15°C to -25°C. |
描述 |
The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. This particular kit also includes 5 tubes of RNase inhibitor (200 µL each).
The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.
Features of this kit include:
• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• 10-fold greater dynamic range than other kits
Extensively tested with a variety of templates
Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.
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